( B) Flat-mount images of embryos are oriented with anterior on the left and posterior on the right. ( A) Wild-type embryos exhibiting expression of glcci1, kiaa0650, krcp in blood cells in the posterior mesoderm at five somites (dorsal view, left panels), in the intermediate cell mass (ICM) blood cells at 24 hours postfertilization (hpf) (lateral view, middle panels), and in circulating blood cells in the vessels and over the yolk (arrowheads, lateral view, right panels). Virtually no expression of these genes was detected in the blood, kidney, or thymus between 48 hpf and 5 dpf although modest levels of kiaa0650 were found in the ventral tail region between 3 and 5 dpf in a pattern similar to scl (data not shown) [įigure 1 glcci1, kiaa0650, krcp are expressed in erythroid blood cell precursors. krcp was expressed in the somites and the tailbud from early somitogenesis to 24 hpf, and after 36 hpf, krcp transcripts were found in the fin buds and in the gut endoderm ( Fig. 1 and data not shown). Diffuse expression of glcci1, kiaa0650 and krcp was observed in regions of the brain at 24 hpf and 36 hpf ( Fig. 1). krcp is also expressed at these stages, but its expression is decreased in the ICM after 20 hpf and found in fewer circulating blood cells than glcci1 or kiaa0650. glcci1 and kiaa0650 are expressed in the circulating primitive blood precursors until 36 to 48 hpf ( Fig. 1, arrowheads). Expression of all three genes is maintained in the blood cells as they migrate medially to form the ICM at 24 hpf ( Fig. 1). A time-course analysis indicated that kiaa0650 and glcci1 are first expressed in blood precursors at the three-somite stage, while krcp transcripts are not detected until the five somites. Further evaluation of glcci1, kiaa0650, and krcp revealed that these genes are expressed in two bilateral stripes of the posterior mesoderm at early stages of somitogenesis ( Fig. 1).
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